The Amur Vaginal Microbiota Panel A, v1.0 is design for qualitative multiplex in-vitro nucleic acid amplification to profile various vaginal microbiota as well as a few common antibiotic resistance genes. DNA from microbes is generally detectable by samples from swabs during the acute phase of infection. Samples are commonly stored in microbial transport media (MTM).
See table below, or the Amplification Master Mix product page for recommended Amplification kits.
HSV2
HSV1
Treponema pallidum
Gardnerella vaginalis
Neisseria gonorrhoeae
Chlamydia trachomatis
Trichomonas vaginalis
Haemophilus ducreyi
Mycoplasma genitalium
Ureaplasma urealyticum
Atopobium vaginae
Group B Strep
Escherichia coli
Enterococcus faecalis
Prevotella bivia
Staphylococcus aureus
Bacteroides fragilis
Mycoplasma hominis
BVAB2
Lactobacillus crispatus
Lactobacillus jensenii
Lactobacillus gasseri
Megasphera 1
Megasphera 2
Mobiluncus curtisii
Mobiluncus mulleris
Candida krusei
Candida tropicalis
Candida galabrata
Candida parapsilosis
Candida albicans
Candida lusitania
Candida auris
VanA (antibiotic resistance gene)
MecA (antibiotic resistance gene)
VanB (antibiotic resistance gene)
Human RNaseP (hRPP)
34 common vaginal microbiota and 3 common antibiotic resistance genes
Extracted Nucleic Acid
FAM
HEX
Cy5
ROX
Amplification 40 cycles recommended
Compatible with qPCR instruments able to detect FAM, HEX, Cy5, and ROX channel fluorescence
Compatible with DNA extraction methods designed to extract high quality DNA from bacterial, viral and fungal targets from human urine or swab samples. Performance to be verified by individual user.
Proprietary assay design providing superior sensitivity and specificity for each target.
Primer/Probe Mix
Positive and Negative Controls
40 cycles using default ramp rates.
Enzyme Activation 95°C 2 min
Amplification 40 cycles (95°C 3s, 59°C 30s)
| Product Name | SKU# | Samples | Storage |
|---|---|---|---|
| Amur Vaginal Microbiota Panel A, v1.0 | LWH1.0-PA-48 | 48 | -20°C |
| Product Name | SKU# | Reactions | Storage |
|---|---|---|---|
| SeqOnce Amplification Kit | SQAK1-400 | 400 | -20°C |
| SeqOnce PLUS Amplification Kit | SQPAK-400 | 400 | -20°C |
For more information see the Amplification Master Mix page.
