Optimized Hi-Performance Enzymes

SeqOnce Amplification Master Mixes

Enzymes and buffers

SeqOnce master mixes and reagents accurately and efficiently amplify nucleic acid from your sample. Just add your SeqOnce probe and primer panel with your sample and the Master Mix and the reaction is ready to go. All master mixes feature HotStart DNA polymerase that remains completely inactive at room-temperature and is activated after only 2 minutes at 95°C. Master mixes with reverse transcriptase utilize a modified version of M-MLV reverse transcriptase with RNAse H activity deactivated and have increased thermal stability.

For Research Use Only. Not intended for in-vitro diagnostics. Not approved by the FDA.

Included in Amplification Kits

All amplification kits include nucleotides, buffers, and reagent-grade H2O and are compatible with SeqOnce primer and probe panels.

Made in America. Research Use Only.

CONTENT

All amplification master mixes include nucleotides, buffers, and reagent-grade H2O.

COMPATIBILITY

Compatible with SeqOnce primer and probe panels for viral and microbiota nucleic acid amplification.

OPTIMIZATION

Optimized for SeqOnce primer and probe panels.

LYOPHILIZATION

Lyophilized amplification master mixes can be manufactured on request.

Ordering

Product Name	SKU#	Description	Reactions	Storage
SeqOnce Amplification Kit	SQAK1-200	Hot Start Taq polymerase Master Mix	200	-20°C
SeqOnce PLUS Amplification Kit	SQPAK-200	HotStart Taq polymerase Master Mix with dUTP/UDG to reduce carry-over contamination risk	200	-20°C
SeqOnce RT-qPCR Amplification Kit	SQRTAK-200	Reverse transcriptase and HotStart Taq polymerase Master Mix	200	-20°C
SeqOnce PLUS RT-qPCR Amplification Kit	SQPRTAK-200	Reverse transcriptase and HotStart Taq polymerase Master Mix with dUTP/UDG to reduce carry-over contamination risk	200	-20°C
SeqOnce OneStep RT-qPCR Amplification Kit	SQOSAK-200	One-step reverse transcriptase in HotStart Taq polymerase Master Mix	200	-20°C
SeqOnce PLUS OneStep RT-qPCR Amplification Kit	SQPOSAK-200	One-step reverse transcriptase in HotStart Taq polymerase Master Mix with dUTP/UDG to reduce carry-over contamination risk	200	-20°C

Order separately: Compatible primer and probe panels

Product Name	SKU#	Reactions	Storage
AmphiSense Respiratory Panel A, v1.0	SQCFDK-200	200	-20°C
AmphiSense Respiratory Panel B, v1.0	SQCFRB-200	200	-20°C
AmphiSense SARS-CoV-2 Panel A, v1.0	SQCVDK-200	200	-20°C
Lioness UT Microbiota Panel A, v2.0	LUTI-PA-16	16 (96 well) or 32 (384 well)	-20°C
Amur ST Microbiota Panel A, v1.0	ASTI-PA-16	16 (96 well) or 32 (384 well)	-20°C
Pongo Monkeypox Panel A, v1.0	ASMPXV-200	200	-20°C
Pongo Monkeypox Panel A, v1.0	ASMPXV-50	50	-20°C