Fastest NGS Library Kit on the Market


DNA Library Kit

The fastest DNA library kit on the market cuts hours off your prep time. Start with as low as 10 ng of material and be ready for sequencing or amplification in 20 minutes. Using a novel, simple three-step procedure, your DNA is enzymatically fragmented without GC bias. Results are comparable to other commercial kits but requires less than 10% of the time.

SeqOnce’s proprietary technology uses a unique set of enzymatic reactions to reduce reaction steps and reagents. The result is a sample preparation kit that is fast, easy to use, and produces high-quality libraries regardless of sample source or application.

For Research Use Only. Not intended for in-vitro diagnostics.

Fast- 20 min Library Prep

Turbocharge your Illumina sequencer. Get faster results. Get higher throughput.

Ultra Rapid

20 min without PCR

40 min with PCR


Single tube

3 steps

No shearing needed


Less hands-on time


8, 24 and 96 wells

Made in America. Research Use Only.

Whole Genome Sequencing (WGS)

Whole Exome Sequencing (WES)

Formalin-fixed, paraffin-embedded (FFPE) Sequencing

Start with as low as 10 ng DNA

Supports size selection >180bp, >300 bp, and dual-size

Stock adapter, fragment reagent, master reagents, and dilution buffer.

D5/D7 dual indices included in 8 well kit and sold separately in 24 and 96 well kits.

Product Name SKU# Reactions Price
RhinoSeq 8 well N/A 8 $160
RhinoSeq 24 well N/A 24 $456
RhinoSeq 96 well N/A 96 $1,632
D5/D7 Indicies 8 well N/A 8 Free (Limit 1)
D5/D7 Indicies 24 well N/A 24 $96
D5/D7 Indicies 96 well N/A 96 $384