The Lioness UT Microbiota Panel is designed for qualitative single-plex in-vitro assessment of common urinary tract microbiota using nucleic acid amplification. Following nucleic acid extraction, the targets are amplified by real-time PCR (qPCR) and detected via fluorescent dye (FAM). The panel is provided in plate format with each probe/primer target in one well.
Positive controls included. Amplification reagents not included. See table below, or the Amplification Master Mix product page for recommended Amplification kits.
Acinetobacter baumannii
Klebsiella aerogenes
Enterobacter cloacae
Enterococcus faecium
Escherichia coli
Klebsiella pneumoniae
Proteus vulgaris
Pseudomonas aeruginosa
Streptococcus agalactiae (gr B)
Serratia marcescens
Mycoplasma hominis
Ureaplasma urealyticum
Staphylococcus aureus
Staphylococcus saprophyticus
Proteus mirabilis
Enterococcus faecalis
Klebsiella Oxytoca
Morganella morganii
Citrobacter freundii
Providencia stuartii
Candida albicans
Candida glabrata
Candida tropicalis
Human RNaseP (control)
23 common urinary tract microbiota
Human RNAseP (control)
Extracted DNA from urine or swab
Single channel, FAM
Amplification 40 cycles recommended
Compatible with qPCR instruments able to detect FAM channel fluorescence
Compatible with DNA extraction methods designed to extract high quality DNA from bacterial, viral and fungal targets from human urine or swab samples. Performance to be verified by individual user.
Proprietary assay design providing superior sensitivity and specificity for each target.
Sensitivity of 1 copy per µl for most targets, depending on chosen extraction method.
Recommended:
40 cycles using default ramp rates
Enzyme Activation 95°C 2 min
Amplification 40 cycles (95°C 3s, 60°C 30s)
| Product Name | SKU# | Storage | |
|---|---|---|---|
| Lioness UT Microbiota Panel A, v2.0 | LUTI-PA-16 | 16 (96 well) or 32 (384 well) | -20°C |
| Product Name | SKU# | Reactions | Storage |
|---|---|---|---|
| SeqOnce Amplification Kit | SQAK1-200 | 200 | -20°C |
| SeqOnce PLUS Amplification Kit | SQPAK-200 | 200 | -20°C |
For more information see the Amplification Master Mix page.
