Urinary Tract Microbiota Detection

Urinary Tract Microbiota Panel:
RT-qPCR

qPCR profiling of 24 common urinary tract microbiota

The Lioness Urinary Tract Microbiota Panel A, v3.0 is designed for qualitative multi-plex in-vitro assessment of common urinary tract microbiota using nucleic acid amplification. Following nucleic acid extraction, the targets are amplified by real-time PCR (qPCR) and detected via fluorescent dye. 

Positive controls included. Amplification reagents not included. See table below, or the Amplification Master Mix product page for recommended Amplification kits.

For Research Use Only. Not intended for in-vitro diagnostics. Not approved by the FDA.

Targets

Acinetobacter baumannii

Klebsiella aerogenes

Enterobacter cloacae

Enterococcus faecium

Escherichia coli

Klebsiella pneumoniae

Proteus vulgaris

Pseudomonas aeruginosa

Streptococcus agalactiae (gr B)

Serratia marcescens

Mycoplasma hominis

Ureaplasma urealyticum

Staphylococcus aureus

Staphylococcus saprophyticus

Proteus mirabilis

Enterococcus faecalis

Klebsiella Oxytoca

Morganella morganii

Citrobacter freundii

Providencia stuartii

Candida albicans

Candida glabrata

Candida tropicalis

Human RNaseP (control)

Targets

24 common urinary tract microbiota

Human RNAseP (control)

Samples

Extracted DNA from urine or swab

Dyes

FAM

HEX

Cy5

ROX

Parameters

Amplification 40 cycles recommended

Compatible with qPCR instruments able to detect FAM, HEX, Cy5, and ROX channels

Made in America. Research Use Only.

Compatible with DNA extraction methods designed to extract high quality DNA from bacterial, viral and fungal targets from human urine or swab samples.  Performance to be verified by individual user.

Proprietary assay design providing superior sensitivity and specificity for each target.

Primer/Probe Mix

Positive and Negative Controls

 

Recommended:

40 cycles using default ramp rates

Enzyme Activation 95°C 2 min

Amplification 40 cycles (95°C 3s, 59°C 30s)

Ordering
Product Name SKU# Samples Storage
Lioness UT Microbiota Panel A, v3.0 LUTI3.0-PA-48 48 -20°C
Recommended Products (Not Included)
Product Name SKU# Reactions Storage
SeqOnce Amplification Kit SQAK1-300 300 -20°C
SeqOnce PLUS Amplification Kit SQPAK-300 300 -20°C

For more information see the Amplification Master Mix page.