Wound Infection Microbiota Detection

Wound Panel: RT-qPCR

qPCR differentiation of 30 wound microbiota

The Glyphis Wound Panel A, v1.0 is design for qualitative multiplex in-vitro nucleic acid amplification to profile various microbes found in wounds. DNA from wound microbes is generally detectable by samples from lesion swabs during the acute phase of infection. Samples are commonly stored in microbial transport media (MTM).

See table below, or the Amplification Master Mix product page for recommended Amplification kits.

For Research Use Only. Not intended for in-vitro diagnostics. Not approved by the FDA.


Acinetobacter baumanii

Escherichia coli

Enterococcus faecalis

Klebsiella oxytoca

Klebsiella pneumoniae

Enterobacter cloacae

Citrobacter freundii

Proteus mirabilis

Morganella morganii

Enterobacter (Klebsiella) aerogenes

Enterococcus faecium

Streptococcus Pyogenes (Strep A)

Staphylococcus aureus

Streptococcus agalactiae

Staphylococcus saprophyticus

Serratia marcescens

Candida krusei

Candida tropicalis

Candida galabrata

Candida parapsilosis

Candida albicans

Candida lusitania

Candida auris

Clostridium septicum

Clostridium novyi

Kingella kingae

Bacteroides fragillis

Clostridium perfringens

Proteus vulgaris

Pseudomonas aeruginosa


30 common wound infecting bacteria

Human RNaseP (control)


Extracted Nucleic Acid







Amplification 40 cycles recommended

Compatible with qPCR instruments able to detect FAM, HEX, Cy5, and ROX channel fluorescence

Made in America. Research Use Only.

Compatible with DNA extraction methods designed to extract high quality DNA from bacterial, viral and fungal targets from human urine or swab samples.  Performance to be verified by individual user.

Proprietary assay design providing superior sensitivity and specificity for each target.

Primer/Probe Mix

Positive and Negative Controls

40 cycles using default ramp rates. 

Enzyme Activation 95°C 2 min

Amplification 40 cycles (95°C 3s, 59°C 30s)

Product Name SKU# Samples Storage
Glyphis Wound Panel A v1.0 WPA1.0-PA-48 48 -20°C
Recommended Products (Not Included)
Product Name SKU# Reactions Storage
SeqOnce Amplification Kit SQAK1-400 400 -20°C
SeqOnce PLUS Amplification Kit SQPAK-400 400 -20°C

For more information see the Amplification Master Mix page.