Sexually Transmitted Microbiota Detection

Sexually Transmitted Microbiota Panel: RT-qPCR

qPCR profiling of 11 common sexually transmitted microbiota

The Amur Sexually Transmitted Microbiota Panel A, v1.0 is designed for qualitative multi-plex in-vitro assessment of common sexually transmitted microbiota using nucleic acid amplification. Following nucleic acid extraction, the targets are amplified by real-time PCR (qPCR) and detected via fluorescent dye. 

Positive controls included. Amplification reagents not included. See table below, or the Amplification Master Mix product page for recommended Amplification kits.

For Research Use Only. Not intended for in-vitro diagnostics. Not approved by the FDA.

Targets

Atopobium vaginae

Gardnerella vaginalis

Herpes Simplex Virus 1

Herpes Simplex Virus 2

Mycoplasma genitalium

Neisseria gonorrhoeae

Chlamydia trachomatis

Haemophilus ducreyi

Trichomonas vaginalis

Treponema pallidium

Ureaplasma urealyticum

Human RNaseP (control)

Targets

11 common sexually transmitted microbiota

Human RNaseP (control)

Samples

Extracted Nucleic Acid

Dyes

FAM

HEX

Cy5

ROX

Parameters

Amplification 40 cycles recommended

Compatible with qPCR instruments able to detect FAM, HEX, Cy5, and ROX channel fluorescence

Made in America. Research Use Only.

Compatible with DNA extraction methods designed to extract high quality DNA from bacterial, viral and fungal targets from human urine or swab samples.  Performance to be verified by individual user.

Proprietary assay design providing superior sensitivity and specificity for each target.

Primer/Probe Mix

Positive and Negative Controls

Recommended:

40 cycles using default ramp rates

Enzyme Activation 95°C 2 min

Amplification 40 cycles (95°C 3s, 58°C 30s)

Ordering
Product Name SKU# Samples Storage
Amur ST Microbiota Panel A, v1.0 PSTI1.0-PA-48 48 -20°C
Recommended Products (Not Included)
Product Name SKU# Reactions Storage
SeqOnce Amplification Kit SQAK1-200 200 -20°C
SeqOnce PLUS Amplification Kit SQPAK-200 200 -20°C

For more information see the Amplification Master Mix page.