Sexually Transmitted Microbiota Detection

Amur ST Microbiota Panel A, v1.0

qPCR profiling of 11 common sexually transmitted microbiota

The Amur ST Microbiota Panel A is designed for qualitative single-plex in-vitro assessment of  common sexually transmitted microbiota using nucleic acid amplification. Following nucleic acid extraction, the targets are amplified by real-time PCR (qPCR) and detected via fluorescent dye (FAM). The panel is provided in plate format with each probe/primer target in one well. 

Positive controls included. Amplification reagents not included. SeqOnce recommends SeqOnce Amplification Kit 1 or SeqOnce PLUS Amplification Kit.

For Research Use Only. Not intended for in-vitro diagnostics. Not approved by the FDA.

Targets

Atopobium vaginae

Gardnerella vaginalis

Herpes Simplex Virus 1

Herpes Simplex Virus 2

Mycoplasma genitalium

Neisseria gonorrhoeae

Chlamydia trachomatis

Haemophilus ducreyi

Trichomonas vaginalis

Treponema pallidium

Ureaplasma urealyticum

Human RNAseP (control)

Targets

11 common sexually transmitted microbiota

Human RNAseP (control)

Samples

Extracted DNA from urine or swab

Dyes

Single channel, FAM

Parameters

Amplification 40 cycles recommended

Compatible with qPCR instruments able to detect FAM channel fluorescence

Made in America. Research Use Only.

Compatible with DNA extraction methods designed to extract high quality DNA from bacterial, viral and fungal targets from human urine or swab samples.  Performance to be verified by individual user.

Proprietary assay design providing superior sensitivity and specificity for each target.

Sensitivity of 1 copy per µl for most targets, depending on chosen extraction method.

Recommended:

40 cycles using default ramp rates

Enzyme Activation 95°C 2 min

Amplification 40 cycles (95°C 3s, 60°C 30s)

Ordering
Product Name SKU# Reactions Storage
Amur ST Microbiota Panel A, v1.0 ASTI-PA-16 16 (96 well) or 32 (384 well) -20°C
Recommended Products (Not Included)
Product Name SKU# Reactions Storage
SeqOnce Amplification Kit 1 SQAK1-200 200 -20°C
SeqOnce PLUS Amplification Kit SQPAK-200 200 -20°C

For more information see the Amplification Master Mix page.