Single-Tube RT-qPCR Detection

AmphiSense Respiratory Panel A, v1.0

RT-qPCR of SARS-CoV-2, Influenza A, Influenza B

The AmphiSense™ Respiratory Panel A is an RT-qPCR assay designed for qualitative multiplex in-vitro assessment of  SARS-CoV-2, Influenza A, and Influenza B. With select viral transport media, no extraction is required- saving both time and cost. Compatible with a wide range of thermal cyclers and robotic liquid handlers.

Positive and negative controls included. Amplification reagents not included. See table below, or the Amplification Master Mix product page for recommended Amplification kits.

For Research Use Only. Not intended for in-vitro diagnostics. Not approved by the FDA.

Same Reagents, Two Workflows

Direct: Select VTM/UTM, no extraction required

Extracted: Conventional magnetic bead extraction

Targets

SARS-CoV-2, N1

Influenza A, M1

Influenza B, NS2

Human RNAseP (control)

Samples

5 µl of Medschenker STM, CDC Media, Hardy VTM, Copan eSwab

Or

5µl extracted RNA (100 ng to 1 pg) 

Dyes

FAM

Quasar 670 (detect in Cy5)

HEX

ROX

Parameters

Amplification 40 cycles recommended

Bio-Rad CFX96/384
Roche LightCycler 480 System
ABI QuantStudio 3, 5, and 7 Pro

Made in America. Research Use Only.

Direct: Heat 100 µl VTM/UTM 95°C for 5 min

Extracted: Approved RNA extraction protocol

CDC recommended targets and sequences

Direct:

SARS-CoV-2:
8-16 copies/µl 

Influenza A:
4-9 copies/µl

Influenza B:
16-33 copies/µl

Recommended:

40 cycles using default ramp rates. Total cycling time approximately 70 sec.

1. RT Incubation 50°C 15 min

2. Enzyme Activation 95°C 2 min

3. Amplification: 40 cycles (95°C 3s, 60°C 30s)

Ordering
Product Name SKU# Reactions Storage
AmphiSense Respiratory Panel A, v1.0 SQCFDK-200 200 -20°C
Recommended Products (Not Included)
Product Name SKU# Reactions Storage
SeqOnce RT-qPCR Amplification Kit SQRTAK-200 200 -20°C
SeqOnce PLUS RT-qPCR Amplification Kit SQPRTAK-200 200 -20°C
SeqOnce OneStep RT-qPCR Amplification Kit SQOSAK-200 200 -20°C
SeqOnce PLUS OneStep RT-qPCR Amplification Kit SQPOSAK-200 200 -20°C

For more information see the Amplification Master Mix page.