The Pongo Antibiotic Resistance Panel Panel A, v2.0 is design for qualitative multiplex in-vitro nucleic acid amplification to profile various antibiotic resistance genes found in microbiota. DNA from microbes is generally detectable by samples from swabs during the acute phase of infection. Samples are commonly stored in microbial transport media (MTM).
See table below, or the Amplification Master Mix product page for recommended Amplification kits.
ACT
ampC Gene
Fox
mecA
qnrB
qnrA
qnrS
vanA1_VanA2
vanB
NDM-1
KPC, bla-KPC
OXA-48, bla-OXA
VIM, bla-VIM
IMP-7, bla-IMP
SHV
TEM
CTX-M Group 1
CTX-M Group 2
CTX-M Group 9
ErmA
ErmB
SUL1
SUL2
Tet
24 common antibiotic resistance genes found in microbiota
Extracted Nucleic Acid
FAM
HEX
Cy5
ROX
Amplification 40 cycles recommended
Compatible with qPCR instruments able to detect FAM, HEX, Cy5, and ROX channel fluorescence
Compatible with DNA extraction methods designed to extract high quality DNA from bacterial, viral and fungal targets from human urine or swab samples. Performance to be verified by individual user.
Proprietary assay design providing superior sensitivity and specificity for each target.
Primer/Probe Mix
Positive and Negative Controls
40 cycles using default ramp rates.
Enzyme Activation 95°C 2 min
Amplification 40 cycles (95°C 3s, 59°C 30s)
| Product Name | SKU# | Samples | Storage |
|---|---|---|---|
| Pongo Antibiotic Resistance Panel A v.2.0 | PAR2.0-PA-48 | 48 | -20°C |
| Product Name | SKU# | Reactions | Storage |
|---|---|---|---|
| SeqOnce Amplification Kit | SQAK1-300 | 300 | -20°C |
| SeqOnce PLUS Amplification Kit | SQPAK-300 | 300 | -20°C |
For more information see the Amplification Master Mix page.
