Antibiotic Resistance Gene Detection

Pongo Antibiotic Resistance Panel: RT-qPCR

qPCR differentiation of 24 antibiotic resistance genes

The Pongo Antibiotic Resistance Panel Panel A, v2.0 is design for qualitative multiplex in vitro nucleic acid amplification to profile various antibiotic resistance genes found in microbiota. DNA from microbes is generally detectable by samples from swabs during the acute phase of infection. Samples are commonly stored in microbial transport media (MTM).

See table below, or the Amplification Master Mix product page for recommended Amplification kits.

For Research Use Only. Not intended for in vitro diagnostics. Not approved by the FDA.

Targets

ACT

ampC Gene

Fox

mecA

qnrB

qnrA

qnrS

vanA1_VanA2

vanB

NDM-1

KPC, bla-KPC

OXA-48, bla-OXA

VIM, bla-VIM

IMP-7, bla-IMP

SHV

TEM

CTX-M Group 1

CTX-M Group 2

CTX-M Group 9

ErmA

ErmB

SUL1

SUL2

Tet

Targets

24 common antibiotic resistance genes found in microbiota

Sample

Extracted Nucleic Acid

Dyes

FAM

HEX

Cy5

ROX

Parameters

Amplification 40 cycles recommended

Compatible with qPCR instruments able to detect FAM, HEX, Cy5, and ROX channel fluorescence

Made in America
Research Use Only

SAMPLE PROCESSING

Compatible with DNA extraction methods designed to extract high quality DNA from bacterial, viral and fungal targets from human urine or swab samples. Performance to be verified by individual user.

PROBES & PRIMERS

Proprietary assay design providing superior sensitivity and specificity for each target.

KIT COMPONENTS

Primer/Probe Mix

Positive and Negative Controls

AMPLIFICATION

40 cycles using default ramp rates.

Enzyme Activation 95°C 2 min

Amplification 40 cycles (95°C 3s, 59°C 30s)

Ordering

Product Name	SKU#	Samples	Storage
Pongo Antibiotic Resistance Panel A	500003	48	-20°C

Product Name	SKU#	Reactions	Storage
SeqOnce Amplification Kit	SQAK1-300	300	-20°C
SeqOnce PLUS Amplification Kit	SQPAK-300	300	-20°C