The Lioness Urinary Tract Microbiota Panel A is designed for qualitative multiplex in vitro detection of common urinary tract pathogens using nucleic acid amplification. Following nucleic acid extraction, the targets are amplified by real-time PCR (qPCR) and detected via a fluorescent dye. The panel consists of the 20 most common bacterial species and 4 yeast species associated with UTIs. When combined with the SeqOnce Pongo antibiotic resistance gene panel it provides a quick and comprehensive assessment of disease status.
Positive controls included. Amplification reagents sold separately, see table below or contact us for more information.
Acinetobacter baumannii
Klebsiella aerogenes
Enterobacter cloacae
Enterococcus faecium
Escherichia coli
Klebsiella pneumoniae
Proteus vulgaris
Pseudomonas aeruginosa
Streptococcus agalactiae (gr B)
Serratia marcescens
Mycoplasma hominis
Ureaplasma urealyticum
Staphylococcus aureus
Staphylococcus saprophyticus
Proteus mirabilis
Enterococcus faecalis
Klebsiella Oxytoca
Morganella morganii
Citrobacter freundii
Providencia stuartii
Candida albicans
Candida glabrata
Candida tropicalis
Candida parapsilosis
Human RNaseP (hRPP)
Compatible with DNA extraction methods designed to extract high quality DNA from bacterial, viral and fungal targets from human urine or swab samples. Performance to be verified by individual user.
Multiplex panel consists of 8 individual tubes with targets in FAM, HEX, Cy5, and ROX fluorophores.
Contact us to request Product Information Sheets for details.
Primer and Probe Mix (8 tubes)
Positive Controls (3 tubes)
Amplification reagents sold separately
Recommended:
SeqOnce qPCR Multiplex Amplification Kit 3000 – PN 500023
Compatible with qPCR instruments capable of detection in FAM, HEX, Cy5, and ROX fluorescence channels (or equivalent excitation / emission wavelengths).
Including but not limited to:
Bio-Rad CFX96/384, and Opus
Roche LightCycler 480 System
ABI QuantStudio 3, 5, 6, 7 and 12 and 7500 series
| Product Name | SKU# | Samples | Storage |
|---|---|---|---|
| Lioness UT Microbiota Panel A | 500012 | 48 | -20°C |
| Product Name | SKU# | Contents | Storage |
|---|---|---|---|
| SeqOnce qPCR Multiplex Amplification Kit 3000 | 500023 | 5 tubes of master mix | -20°C |
| 1 tube of nuclease free water |
For more information see the Amplification Master Mix page.
