In response to increasing H5N1 bird flu concerns, SeqOnce Biosciences has developed a robust and versatile, single tube assay to screen both human and veterinary samples.
The H5N1 Avian Flu Panel is an RT-qPCR assay designed for qualitative, single tube multiplex in vitro assessment of nucleic acid from the H5 (Hemagglutinin 5 (HA) gene) and Influenza A M1 gene. The H5 gene is designed to detect all known clades and variants of highly pathogenic avian Influenza (HPAI) A/H5N1, including the most recent human infectious strains. The Influenza A assay has been shown to detect all known Hemagglutinin (HA) and Neuraminidase (NA) (H*N*) flu A subtypes via a highly conserved region of the M1 gene. The internal control is a proprietary multi species control that detects nucleic acid from most higher vertebrate species, including human, bovine, and avian DNA.
Compatible with a wide range of thermal cyclers and robotic liquid handlers.
For your convenience our optimized Amplification kits are available to be bundled with our panels and are listed at the bottom of this page.
| Recommended interpretation | Pathogen Pan Flu (HEX) | Pathogen Pan Flu (HEX) | Pathogen Pan Flu (HEX) | |||
|---|---|---|---|---|---|---|
| Pathogen Pan Flu (HEX) | Pathogen H5 gene (FAM) | SPRY1 Internal Control (ROX) | Result Interpretation | |||
| - | - | - | Invalid | |||
| - | - | + | Pathogen Nucleic Acid not detected | |||
| - | + | +/- | Inconclusive | |||
| + | + | +/- | Influenza H5 specific detected | |||
| + | - | +/- | Influenza A – Not H5 detected | |||
4 µL sample eluate from any nucleic acid extraction method that produces high quality DNA and RNA from desired sample type.
Performance to be verified and validated by laboratory.
H5 (Hemagglutinin 5 (HA) gene) – FAM
Influenza A (M1 gene) – HEX
Multispecies internal control – ROX
See our product information sheet for more information
Primer and probe mix
Proprietary synthetic control
Amplification reagents sold separately
Recommended: SeqOnce OneStep RT-qPCR Multiplex Amplification Kit 1800 – PN 500030
40 cycles using default ramp rates. Total cycling time approximately 70 minutes
Compatible with qPCR instruments capable of detection in FAM, HEX, and ROX fluorescence channels (or equivalent excitation / emission wavelengths).
Including but not limited to:
Bio-Rad CFX96/384, and Opus
Roche LightCycler 480 System
ABI QuantStudio 3, 5, 6, 7 and 12 and 7500 series
| Product Name | SKU# | Reactions | Storage | Price |
|---|---|---|---|---|
| H5N1 Bird Flu Panel Kit, Enzyme, and water bundle | 500029 & 500030 | 200 | -20°C |
$1,100 $900 |
| H5N1 Bird Flu Panel | 500029 | 200 | -20°C | $275 |
| SeqOnce OneStep RT-qPCR Multiplex Amplification Kit 1800 | 500030 | 200 | -20°C | $825 |
For more information see the Amplification Master Mix page.
