In response to increasing H5N1 bird flu concerns, SeqOnce Biosciences has developed a robust and versatile, single tube assay to screen both human and veterinary samples.
The H5N1 Avian Flu Panel is an RT-qPCR assay designed for qualitative, single tube multiplex in vitro assessment of nucleic acid from the H5 (Hemagglutinin 5 (HA) gene) and Influenza A M1 gene. The H5 gene is designed to detect all known clades and variants of highly pathogenic avian Influenza (HPAI) A/H5N1, including the most recent human infectious strains. The Influenza A assay has been shown to detect all known Hemagglutinin (HA) and Neuraminidase (NA) (H*N*) flu A subtypes via a highly conserved region of the M1 gene. The internal control is a proprietary multi species control that detects nucleic acid from most higher vertebrate species, including human, bovine, and avian DNA.
Compatible with a wide range of thermal cyclers and robotic liquid handlers.
Positive control included. Amplification reagents sold separately, see table below or contact us for more information.
| Recommended interpretation | Pathogen Pan Flu (HEX) | Pathogen Pan Flu (HEX) | Pathogen Pan Flu (HEX) | |||
|---|---|---|---|---|---|---|
| Pathogen Pan Flu (HEX) | Pathogen H5 gene (FAM) | SPRY1 Internal Control (ROX) | Result Interpretation | |||
| - | - | - | Invalid | |||
| - | - | + | Pathogen Nucleic Acid not detected | |||
| - | + | +/- | Inconclusive | |||
| + | + | +/- | Influenza H5 specific detected | |||
| + | - | +/- | Influenza A – Not H5 detected | |||
4 µL sample eluate from any nucleic acid extraction method that produces high quality DNA and RNA from desired sample type.
Performance to be verified and validated by laboratory.
H5 (Hemagglutinin 5 (HA) gene) – FAM
Influenza A (M1 gene) – HEX
Multispecies internal control – ROX
Primer and probe mix
Proprietary synthetic control
Amplification reagents sold separately
Recommended: SeqOnce OneStep RT-qPCR Multiplex Amplification Kit 1800 – PN 500030
40 cycles using default ramp rates. Total cycling time approximately 70 minutes
Compatible with qPCR instruments capable of detection in FAM, HEX, and ROX fluorescence channels (or equivalent excitation / emission wavelengths).
Including but not limited to:
Bio-Rad CFX96/384, and Opus
Roche LightCycler 480 System
ABI QuantStudio 3, 5, 6, 7 and 12 and 7500 series
| Product Name | SKU# | Samples | Storage |
|---|---|---|---|
| H5N1 Bird Flu Panel | 500029 | 200 | -20°C |
| Product Name | SKU# | Contents | Storage |
|---|---|---|---|
| SeqOnce OneStep RT-qPCR Multiplex Amplification Kit 1800 | 500030 | 3 tubes of master mix | -20°C |
| 1 tube of nuclease free water |
For more information see the Amplification Master Mix page.
