Single-Tube RT-qPCR Detection

SARS-CoV-2, Influenza A & B, RSV:
RT-qPCR

Q: SAMPLE PROCESSING

Direct : Heat 100 µl VTM/UTM 95°C for 5 min Extracted : Approved RNA extraction protocol Performance to be verified and validated by laboratory.

Q: KIT COMPONENTS

Direct: SARS-CoV-2: 8-16 copies/µl Influenza A: 4-9 copies/µl Influenza B: 16-33 copies/µl RSV: 100 copies/µl

Q: AMPLIFICATION

Recommended: 40 cycles using default ramp rates. Total cycling time approximately 70 sec. 1. RT Incubation 50°C 15 min 2. Enzyme Activation 95°C 2 min 3. Amplification: 40 cycles (95°C 3s, 60°C 30s)

Amphisense Respiratory Panel B, v1.0

The AmphiSense™ Respiratory Panel B is an RT-qPCR assay designed for qualitative multiplex in-vitro assessment of SARS-CoV-2, Influenza A, Influenza B and Respiratory Syncytial Virus (RSV) nucleic acid. With select viral transport media, no extraction is required- saving both time and cost. Compatible with a wide range of thermal cyclers and robotic liquid handlers.

Positive and negative controls included. Amplification reagents not included. See table below, or the Amplification Master Mix product page for recommended Amplification kits.

For Research Use Only. Not intended for in-vitro diagnostics. Not approved by the FDA.

Same Reagents, Two Workflows

Direct: Select VTM/UTM, no extraction required

Extracted: Conventional magnetic bead extraction

Targets

SARS-CoV-2, N1

Influenza A, M1

Influenza B, NS2

RSV, Matrix

Human RNaseP (control)

Samples

5 µl of Medschenker STM, CDC Media, Hardy VTM, Copan eSwab

5µl extracted RNA (100 ng to 1 pg)

Dyes

FAM

Quasar 670 (detect in Cy5)

Quasar 705 (detect in Cy5.5)

HEX

ROX

Parameters

Amplification 40 cycles recommended

Made in America. Research Use Only.

SAMPLE PROCESSING

Direct: Heat 100 µl VTM/UTM 95°C for 5 min

Extracted: Approved RNA extraction protocol

Performance to be verified and validated by laboratory.

PROBES & PRIMERS

SARS-CoV-2, Influenza A, Influenza B, CDC recommended targets and sequences. Pan RSV primer/probe Thongpan et al., 2019.

KIT COMPONENTS

Direct:

SARS-CoV-2:
8-16 copies/µl

Influenza A:
4-9 copies/µl

Influenza B:
16-33 copies/µl

RSV:
100 copies/µl

AMPLIFICATION

Recommended:

40 cycles using default ramp rates. Total cycling time approximately 70 sec.

1. RT Incubation 50°C 15 min

2. Enzyme Activation 95°C 2 min

3. Amplification: 40 cycles (95°C 3s, 60°C 30s)

Ordering

Product Name	SKU#	Reactions	Storage
AmphiSense Respiratory Panel B, v1.0	SQCFRB-200	200	-20°C

Product Name	SKU#	Reactions	Storage
SeqOnce RT-qPCR Amplification Kit	SQRTAK-200	200	-20°C
SeqOnce PLUS RT-qPCR Amplification Kit	SQPRTAK-200	200	-20°C
SeqOnce OneStep RT-qPCR Amplification Kit	SQOSAK-200	200	-20°C
SeqOnce PLUS OneStep RT-qPCR Amplification Kit	SQPOSAK-200	200	-20°C