Understanding NGS Library Complexity: Sources of Sequencing Errors & Limits of Detection
Next-generation sequencing is, by definition, “massively parallel.” While the first 454/Curagen GS20 systems yielded a million reads per run, the latest sequencing platforms from Illumina, (NovaSeq 6000 running their highest-capacity S4 flowcell), yields 10,000X the number (i.e. 10 billion reads) per sequencing run. Applications such as somatic mutation detection in cancer samples require increasingly greater […]
