Multiplex panel consists of 9 individual tubes with targets in FAM, HEX, Cy5, and ROX fluorophores. Contact us to request Product Information Sheets for details.

Wound Infection Microbiota Detection

Glyphis Wound Panel

Q: AMPLIFICATION

Recommended: SeqOnce qPCR Multiplex Amplification Kit 3600 – PN 500024 Compatible with qPCR instruments capable of detection in FAM, HEX, Cy5, and ROX fluorescence channels (or equivalent excitation / emission wavelengths). Including but not limited to: Bio-Rad CFX96/384, and Opus Roche LightCycler 480 System ABI QuantStudio 3, 5, 6, 7 and 12 and 7500 series

qPCR differentiation of 30 wound microbiota

The Glyphis Wound Panel A is designed for qualitative, multiplex in vitro nucleic acid amplification to detect pathogenic microbes found in infected wounds. This panel includes yeast as well as both aerobic and anaerobic bacteria and gives rapid insight into difficult to grow and slow growing organisms. When combined with the SeqOnce Pongo antibiotic resistance gene panel the combo provides a quick and comprehensive tool to assess time sensitive infections.

Amplification reagents sold separately, see table below or contact us for more information.

For Research Use Only. Not intended for in vitro diagnostics. Not approved by the FDA.

Targets

Acinetobacter baumanii

Escherichia coli

Enterococcus faecalis

Klebsiella oxytoca

Klebsiella pneumoniae

Enterobacter cloacae

Citrobacter freundii

Proteus mirabilis

Morganella morganii

Enterobacter (Klebsiella) aerogenes

Enterococcus faecium

Streptococcus Pyogenes (Strep A)

Staphylococcus aureus

Streptococcus agalactiae

Staphylococcus saprophyticus

Serratia marcescens

Candida krusei

Candida tropicalis

Candida galabrata

Candida parapsilosis

Candida albicans

Candida lusitania

Candida auris

Clostridium septicum

Clostridium novyi

Kingella kingae

Bacteroides fragillis

Clostridium perfringens

Proteus vulgaris

Pseudomonas aeruginosa

Human RNaseP (hRPP)

Made in America
Research Use Only

SAMPLE PROCESSING

Compatible with DNA extraction methods designed to extract high quality DNA from bacterial, viral and fungal targets from human urine or swab samples. Performance to be verified by individual user.

PROBES & PRIMERS

Multiplex panel consists of 9 individual tubes with targets in FAM, HEX, Cy5, and ROX fluorophores.

KIT COMPONENTS

Primer and Probe Mix (9 tubes)

Positive Controls (4 tubes)

Amplification reagents sold separately

AMPLIFICATION

Recommended: SeqOnce qPCR Multiplex Amplification Kit 3600 – PN 500024

Compatible with qPCR instruments capable of detection in FAM, HEX, Cy5, and ROX fluorescence channels (or equivalent excitation / emission wavelengths).

Including but not limited to:

Bio-Rad CFX96/384, and Opus

Roche LightCycler 480 System

ABI QuantStudio 3, 5, 6, 7 and 12 and 7500 series

Ordering

Product Name	SKU#	Samples	Storage
Glyphis Wound Panel A	500002	48	-20°C

Product Name	SKU#	Contents	Storage
SeqOnce qPCR Multiplex Amplification Kit 3600	500024	6 tubes of master mix	-20°C
SeqOnce qPCR Multiplex Amplification Kit 3600	500024		-20°C		2 tubes of nuclease free water