The Glyphis Wound Panel A, v1.0 is design for qualitative multiplex in-vitro nucleic acid amplification to profile various microbes found in wounds. DNA from wound microbes is generally detectable by samples from lesion swabs during the acute phase of infection. Samples are commonly stored in microbial transport media (MTM).
See table below, or the Amplification Master Mix product page for recommended Amplification kits.
Acinetobacter baumanii
Escherichia coli
Enterococcus faecalis
Klebsiella oxytoca
Klebsiella pneumoniae
Enterobacter cloacae
Citrobacter freundii
Proteus mirabilis
Morganella morganii
Enterobacter (Klebsiella) aerogenes
Enterococcus faecium
Streptococcus Pyogenes (Strep A)
Staphylococcus aureus
Streptococcus agalactiae
Staphylococcus saprophyticus
Serratia marcescens
Candida krusei
Candida tropicalis
Candida galabrata
Candida parapsilosis
Candida albicans
Candida lusitania
Candida auris
Clostridium septicum
Clostridium novyi
Kingella kingae
Bacteroides fragillis
Clostridium perfringens
Proteus vulgaris
Pseudomonas aeruginosa
30 common wound infecting bacteria
Human RNaseP (control)
Extracted Nucleic Acid
FAM
HEX
Cy5
ROX
Amplification 40 cycles recommended
Compatible with qPCR instruments able to detect FAM, HEX, Cy5, and ROX channel fluorescence
Compatible with DNA extraction methods designed to extract high quality DNA from bacterial, viral and fungal targets from human urine or swab samples. Performance to be verified by individual user.
Proprietary assay design providing superior sensitivity and specificity for each target.
Primer/Probe Mix
Positive and Negative Controls
40 cycles using default ramp rates.
Enzyme Activation 95°C 2 min
Amplification 40 cycles (95°C 3s, 59°C 30s)
| Product Name | SKU# | Samples | Storage |
|---|---|---|---|
| Glyphis Wound Panel A v1.0 | WPA1.0-PA-48 | 48 | -20°C |
| Product Name | SKU# | Reactions | Storage |
|---|---|---|---|
| SeqOnce Amplification Kit | SQAK1-400 | 400 | -20°C |
| SeqOnce PLUS Amplification Kit | SQPAK-400 | 400 | -20°C |
For more information see the Amplification Master Mix page.
