The AmphiSense™ Respiratory Panel A is an RT-qPCR assay designed for qualitative multiplex in-vitro assessment of SARS-CoV-2, Influenza A, and Influenza B nucleic acid. With select viral transport media, no extraction is required- saving both time and cost. Compatible with a wide range of thermal cyclers and robotic liquid handlers.
Positive and negative controls included. Amplification reagents not included. See table below, or the Amplification Master Mix product page for recommended Amplification kits.
Direct: Select VTM/UTM, no extraction required
Extracted: Conventional magnetic bead extraction
SARS-CoV-2, N1
Influenza A, M1
Influenza B, NS2
Human RNaseP (control)
5 µl of Medschenker STM, CDC Media, Hardy VTM, Copan eSwab
Or
5µl extracted RNA (100 ng to 1 pg)
FAM
Quasar 670 (detect in Cy5)
HEX
ROX
Amplification 40 cycles recommended
Bio-Rad CFX96/384
Roche LightCycler 480 System
ABI QuantStudio 3, 5, and 7 Pro
Direct: Heat 100 µl VTM/UTM 95°C for 5 min
Extracted: Approved RNA extraction protocol
Performance to be verified and validated by laboratory.
CDC recommended targets and sequences
Direct:
SARS-CoV-2:
8-16 copies/µl
Influenza A:
4-9 copies/µl
Influenza B:
16-33 copies/µl
Recommended:
40 cycles using default ramp rates. Total cycling time approximately 70 sec.
1. RT Incubation 50°C 15 min
2. Enzyme Activation 95°C 2 min
3. Amplification: 40 cycles (95°C 3s, 60°C 30s)
| Product Name | SKU# | Reactions | Storage |
|---|---|---|---|
| AmphiSense Respiratory Panel A, v1.0 | SQCFDK-200 | 200 | -20°C |
| Product Name | SKU# | Reactions | Storage |
|---|---|---|---|
| SeqOnce RT-qPCR Amplification Kit | SQRTAK-200 | 200 | -20°C |
| SeqOnce PLUS RT-qPCR Amplification Kit | SQPRTAK-200 | 200 | -20°C |
| SeqOnce OneStep RT-qPCR Amplification Kit | SQOSAK-200 | 200 | -20°C |
| SeqOnce PLUS OneStep RT-qPCR Amplification Kit | SQPOSAK-200 | 200 | -20°C |
For more information see the Amplification Master Mix page.
