The fastest DNA library kit on the market cuts hours off your prep time. Start with as low as 10 ng of material and be ready for sequencing or amplification in 20 minutes. Using a novel, simple three-step procedure, your DNA is enzymatically fragmented without GC bias. Results are comparable to other commercial kits but requires less than 10% of the time.

Turbocharge your Illumina sequencer. Get faster results. Get higher throughput.

Library Construction in 20′
See the Difference

 SeqOnce BiosciencesIllumina® / NexteraKAPACovaris® Shearing + KAPANEBSwift Biosciences™ /
Time (minutes)20-40150150-180210-240150-180210-270
Workflow ComplexitySimple (3 steps)HighModerateModerateModerateModerate
Sample Input10ng-100ng50ng10ng-5µg1ng-1µg1ng-100ng10pg-250ng
Number of RXN's (formats)8, 24, 9612, 48, 968, 48, 968, 24, 48, 9612, 24, 48, 9624, 96

Ordering Information

DNA library amplification kit for Illumina Sequencers. Applications include WGS, WES, FFPE

Research Use Only


  • 8 well | $20/reaction | $160
  • 24 well | $19/reaction | $456
  • 96 well | $17/reaction | $1,632


  • 8 well D5/D7 indices | Free (Limit 1)
  • 24 well D5/D7 indices |$4/reaction | $96
  • 96 well D5/D7 indices |$4/reaction | $384

Due to Supply Chain Issues This Product is on Backorder

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Download the RhinoSeq DNA Library Prep Kit Protocols

  • Acinetobacter baumannii
  • Klebsiella aerogenes
  • Enterobacter cloacae
  • Enterococcus faecium
  • Escherichia coli
  • Klebsiella pneumoniae
  • Proteus vulgaris
  • Pseudomonas aeruginosa
  • Streptococcus agalactiae (group B)
  • Serratia marcescens
  • Mycoplasma hominis
  • Ureaplasma urealyticum
  • Staphylococcus aureus
  • Staphylococcus saprophyticus
  • Proteus mirabilis
  • Enterococcus faecalis
  • Klebsiella oxytoca
  • Morganella morganii
  • Citrobacter freundii
  • Providencia stuartii
  • Candida albicans
  • Candida glabrata
  • Candida tropicalis

We recommend MagMAX DNA Multi-Sample Ultra Kit.


The RhinoSeq DNA library kit provides the reagents necessary for amplification. PCR cycles can range from 12-15 cycles depending upon DNA input and quality.

The RhinoSeq DNA library kit contains reagents for amplification. A PCR-free version will be available soon.

Starting material should be purified to minimize the presence of contaminants and maximize high molecular weight DNA. Samples should be quantified by a fluorometric method (e.g. Qubit, PicoGreen) prior library construction.

The RhinoSeq DNA library kit provides protocol recommendations for size selection greater than 180bp, 300bp, and dual size selection.

Workflows for these sample types are in development and will be available soon.

SeqOnce kits work with high and low complexity samples. Proper fluorometric quantification (e.g. Qubit, Picogreen) and Bioanalyzer analysis of the samples are recommended prior to library construction to assay sample quality.

The kit comes in 24 and 96 reaction formats. For customized orders, reagents can be drop shipped in bulk format.

Yes, the library prep kit offers fragmentation, library construction, and PCR all in a single tube.

Yes, temporarily, but transitioning quickly between steps is recommended to maximize yields. In fact, the reagents are stable at ambient temperature for up to one week.

Yes, DNA can be stored in buffer with EDTA concentration 0.1-1mM.

Kit is suitable for 10ng – 100ng of input DNA. Lower input is currently in R&D.

The shelf life of the kit is 12 months.

The kits shipped on ice packs or dry ice. All components can be safely stored at -20°C.

SeqOnce currently uses Illumina® Sequencing platforms.

Product Disclaimer

Download MSDS Sheets


Using the RhinoSeq DNA library kit enables more flexibility with our time due to the ease of use and rapid speed. We don’t need to block out an entire day to prepare libraries.

Matthew Collin - Genomics Core Manager, UC Riverside

Generating NGS libraries in under an hour which produce quality sequencing data, has the ability to change the landscape of NGS library prep, allowing researchers a huge amount of flexibility in their day to day workload.

Gary Sommerville, PhD - Sr. Research Scientist, Dana Farber Cancer Institute


By highlighting threatened or endangered species, SeqOnce hopes to draw attention to conservation efforts. As biologists, we believe humanity is better served when ecologies are maintained and there is diversity of life.

SeqOnce supports protecting and conserving endangered and threatened species.

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