The fastest DNA library kit on the market cuts hours off your prep time. Start with as low as 10 ng of material and be ready for sequencing or amplification in 20 minutes. Using a novel, simple three-step procedure, your DNA is enzymatically fragmented without GC bias. Results are comparable to other commercial kits but requires less than 10% of the time.

Turbocharge your Illumina sequencer. Get faster results. Get higher throughput.

Library Construction in 20′
See the Difference

 SeqOnce BiosciencesIllumina® / NexteraKAPACovaris® Shearing + KAPANEBSwift Biosciences™ /
Time (minutes)20-40150150-180210-240150-180210-270
Workflow ComplexitySimple (3 steps)HighModerateModerateModerateModerate
Sample Input10ng-100ng50ng10ng-5µg1ng-1µg1ng-100ng10pg-250ng
Number of RXN's (formats)8, 24, 9612, 48, 968, 48, 968, 24, 48, 9612, 24, 48, 9624, 96

Ordering Information

DNA library amplification kit for Illumina Sequencers. Applications include WGS, WES, FFPE


  • 8 well | $20/reaction | $160
  • 24 well | $19/reaction | $456
  • 96 well | $17/reaction | $1,632


  • 8 well D5/D7 indices | Free (Limit 1)
  • 24 well D5/D7 indices |$4/reaction | $96
  • 96 well D5/D7 indices |$4/reaction | $384

Due to Supply Chain Issues This Product is on Backorder

Contact or Email Us for More Information

Download the RhinoSeq DNA Library Prep Kit Protocols

  • Acinetobacter baumannii
  • Klebsiella aerogenes
  • Enterobacter cloacae
  • Enterococcus faecium
  • Escherichia coli
  • Klebsiella pneumoniae
  • Proteus vulgaris
  • Pseudomonas aeruginosa
  • Streptococcus agalactiae (group B)
  • Serratia marcescens
  • Mycoplasma hominis
  • Ureaplasma urealyticum
  • Staphylococcus aureus
  • Staphylococcus saprophyticus
  • Proteus mirabilis
  • Enterococcus faecalis
  • Klebsiella oxytoca
  • Morganella morganii
  • Citrobacter freundii
  • Providencia stuartii
  • Candida albicans
  • Candida glabrata
  • Candida tropicalis

We recommend MagMAX DNA Multi-Sample Ultra Kit.


No. The controls are ordered as a separate sku and come pre-diluted, ready for use.

Yes- the kit is licensed from Bantek, Inc. which has an authorized license from Yale SalivaDirect™ to manufacture the SalivaDirect assay components.

According to the Yale University website (January 21, 2021):

The SalivaDirect team is currently validating or planning to validate the following PCR instrumentation: AriaMX, CFX384, QuantStudio 5 (96 and 384), QuantStudio 6 (96 and 384), QuantStudio 7 (96 and 384), QuantStudio 12k. The SalivaDirect team is NOT planning to validate otherwise fully automated RNA extraction and PCR platforms. We are prioritizing separate PCR instrumentation as they permit greater flexibility to laboratories including more cost-effective reagents.

  • CFX96 Touch Real-Time PCR Detection System
  • CFX384 Touch Real-Time PCR Detection System
  • Applied Biosystems StepOne Real-Time PCR System
  • Applied Biosystems StepOne Plus Real- Time PCR System
  • Applied Biosystems 7500 Fast Real-Time PCR System
  • Applied Biosystems 7500 Fast Dx Real-Time PCR System
  • ABI QuantStudio 5 Real-Time PCR system (96 or 384 well format)
  • ABI QuantStudio 6 Real-Time PCR system (96 or 384 well format)
  • ABI QuantStudio 7 Pro Real-Time PCR system (96 or 384 well format)
  • ABI QuantStudio 7 Flex Real-Time PCR system (96 or 384 well format)
  • ABI QuantStudio 12K Flex Real-Time PCR system (384 well format)
  • ABI QuantStudio Dx Real-Time PCR system (96 well format)
  • Liberty16
  • Cobas Z480
  • LightCycler 480
  • Open qPCR
  • qTower
  • AriaMX Real-Time PCR System

Yes. Fill out and submit Authorized Laboratory Agreement with Yale University. Learn more about the Designation Process here.


SARS-CoV-2 A(23063)T, G(23012)A, A(22812).

FAM, HEX, ROX/TexasRed

SARS-CoV-2 (G23012A)

No- it is RUO

CDC Media (Charm), Remel-4RT (R12699), Hardy Viral Transport Media (R99), Medschenker STM (STM30-A or STM30), Copan (480C), Puritan UniTranz (UT-100), Puritan Amies (LA-106)


The N1 utilizes CDC primer/probe sequences and is designed to allow the assay to be used both as a primary screening tool (positive/negative) for SARS-CoV-2 and confirmatory of previously positive samples (variant identification).

SARS-CoV-2 A(23063)T

The assay uses standard channels FAM, HEX, Cy5, ROX.


The RhinoSeq DNA library kit provides the reagents necessary for amplification. PCR cycles can range from 12-15 cycles depending upon DNA input and quality.

The RhinoSeq DNA library kit contains reagents for amplification. A PCR-free version will be available soon.

Starting material should be purified to minimize the presence of contaminants and maximize high molecular weight DNA. Samples should be quantified by a fluorometric method (e.g. Qubit, PicoGreen) prior library construction.

The RhinoSeq DNA library kit provides protocol recommendations for size selection greater than 180bp, 300bp, and dual size selection.

Workflows for these sample types are in development and will be available soon.

SeqOnce kits work with high and low complexity samples. Proper fluorometric quantification (e.g. Qubit, Picogreen) and Bioanalyzer analysis of the samples are recommended prior to library construction to assay sample quality.

The kit comes in 24 and 96 reaction formats. For customized orders, reagents can be drop shipped in bulk format.

Yes, the library prep kit offers fragmentation, library construction, and PCR all in a single tube.

Yes, temporarily, but transitioning quickly between steps is recommended to maximize yields. In fact, the reagents are stable at ambient temperature for up to one week.

Yes, DNA can be stored in buffer with EDTA concentration 0.1-1mM.

Kit is suitable for 10ng – 100ng of input DNA. Lower input is currently in R&D.

The shelf life of the kit is 12 months.

The kits shipped on ice packs or dry ice. All components can be safely stored at -20°C.

SeqOnce currently uses Illumina® Sequencing platforms.

Product Disclaimer

Download MSDS Sheets


Using the RhinoSeq DNA library kit enables more flexibility with our time due to the ease of use and rapid speed. We don’t need to block out an entire day to prepare libraries.

Matthew Collin - Genomics Core Manager, UC Riverside

Generating NGS libraries in under an hour which produce quality sequencing data, has the ability to change the landscape of NGS library prep, allowing researchers a huge amount of flexibility in their day to day workload.

Gary Sommerville, PhD - Sr. Research Scientist, Dana Farber Cancer Institute


By highlighting threatened or endangered species, SeqOnce hopes to draw attention to conservation efforts. As biologists, we believe humanity is better served when ecologies are maintained and there is diversity of life.

SeqOnce supports protecting and conserving endangered and threatened species.

Learn More Here


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