Single-Tube qPCR Detection

Amur Monkeypox Panel A, v1.0

qPCR differentiation of Monkeypox Clade I and Clade II

The Amur Monkeypox Panel is design for for qualitative multiplex in-vitro nucleic acid amplification to profile various strains of Monkeypox. DNA from Monkeypox virus is generally detectable by samples from lesion swabs during the acute phase of infection. Samples are commonly stored in viral transport media (VTM).

Negative control included. Positive controls not included. Amplification reagents not included. SeqOnce recommends SeqOnce Amplification Kit 1 or SeqOnce PLUS Amplification Kit.

For Research Use Only. Not intended for in-vitro diagnostics. Not approved by the FDA.

Targets

Detects both West African (Clade II) and Congo Basin (Clade I).

Targets

Monkeypox virus generic (CDC Design), G2R

Monkeypox virus West Africa, G2R

Monkeypox virus generic envelope protein, B6R

Human RNAseP (control)

Samples

Purified DNA from swabs

Dyes

FAM

HEX

Cy5

TxRed

Parameters

Amplification 40 cycles

Made in America. Research Use Only.

Approved DNA extraction protocol

CDC recommended targets and sequences, and proprietary targets and sequences

5-10 copies

40 cycles using default ramp rates. 

Enzyme Activation 95°C 2 min

Amplification 40 cycles (95°C 3s, 58°C 1s)

Ordering
Product Name SKU# Reactions Storage
Amur Monkeypox Panel A, v1.0 ASMPXV-25 25 -20°C
Amur Monkeypox Panel A, v1.0 ASMPXV-200 200 -20°C
Recommended Products (Not Included)
Product Name SKU# Reactions Storage
SeqOnce Amplification Kit 1 SQAK1-200 200 -20°C
SeqOnce PLUS Amplification Kit SQPAK-200 200 -20°C

For more information see the Amplification Master Mix page.