AzureSeq SARS-CoV-2 N501Y
ONE STEP UNIVERSAL RT-qPCR KIT
SARS-CoV-2 N501Y VARIANT DETECTION
Rapidly detect the N501Y variant without sequencing, while greatly increasing the number of samples screened. This assay detects the SARS-CoV-2 nucleocapsid gene (N1) and the S gene (A23063T) variant N501Y. This variant is reported in lineages UK B.1.1.7 , SA B.135.1, and Brazil P.1.
TWO PROTOCOLS
Direct & Extracted
Multiplex – 3 targets per reaction well
Made in America
Research Use Only
Targets
Nucleocapsid and S gene of SARS-CoV-2, RNaseP gene (control)
Sample
100ng to 1pg extracted RNA
or 5ul VTM
(see IFU for VTM compatibility)
Dyes/Cycles
N1= FAM, N501 Wildtype=VIC, N501Y Variant=Cy5, RNaseP=TexasRed. Amplification 40 cycles, LoD 5-10 copies
PERFORMANCE EVALUATION
Contrived Clinical Samples
No- it is RUO/LDT
CDC, Pyramid, Copan, Saline, PBS, Mawi-El
The N1 utilizes CDC primer/probe sequences and is designed to allow the assay to be used both as a primary screening tool (positive/negative) for SARS-CoV-2 and confirmatory of previously positive samples (variant identification).
SARS-CoV-2 A(23063)T
The assay uses standard channels FAM, HEX, Cy5, ROX.