AzureSeq SARS-CoV-2 N501Y
ONE STEP UNIVERSAL RT-qPCR KIT
SARS-CoV-2 N501Y VARIANT DETECTION
Rapidly detect the N501Y variant without sequencing, while greatly increasing the number of samples screened. This assay detects the SARS-CoV-2 nucleocapsid gene (N1) and the S gene (A23063T) variant N501Y. This variant is reported in lineages UK B.1.1.7 , SA B.135.1, and Brazil P.1.
TWO PROTOCOLS
Direct & Extracted
Multiplex – 3 targets per reaction well
Made in America
Research Use Only
Targets
Nucleocapsid and S gene of SARS-CoV-2, RNaseP gene (control)
Sample
100ng to 1pg extracted RNA
or 5ul VTM
(see IFU for VTM compatibility)
Dyes/Cycles
N1= FAM, N501 Wildtype=VIC, N501Y Variant=Cy5, RNaseP=TexasRed. Amplification 40 cycles, LoD 5-10 copies
PERFORMANCE EVALUATION
Contrived Clinical Samples
N501Y Variant
N501Y Variant result. HEX (wild type) shows no amplification, while Quasar 670 (N501Y variant) is amplified.
N501 Wildtype
N501 Wild type result. HEX (wild type) shows amplification, while Quasar 670 (N501Y variant) shows no amplification.
N1
N1 detection and control RNase P
No- it is RUO/LDT
CDC, Pyramid, Copan, Saline, PBS, Mawi-El
The N1 utilizes CDC primer/probe sequences and is designed to allow the assay to be used both as a primary screening tool (positive/negative) for SARS-CoV-2 and confirmatory of previously positive samples (variant identification).
SARS-CoV-2 A(23063)T
The assay uses standard channels FAM, HEX, Cy5, ROX.