AzureSeq SARS-CoV-2 N501Y

ONE STEP UNIVERSAL RT-qPCR KIT
SARS-CoV-2 N501Y VARIANT DETECTION

Rapidly detect the N501Y variant without sequencing, while greatly increasing the number of samples screened. This assay detects the SARS-CoV-2 nucleocapsid gene (N1) and the S gene (A23063T) variant N501Y. This variant is reported in lineages UK B.1.1.7 , SA B.135.1, and Brazil P.1.

TWO PROTOCOLS
Direct & Extracted

Multiplex – 3 targets per reaction well
Made in America
Research Use Only

Targets

Nucleocapsid and S gene of SARS-CoV-2, RNaseP gene (control)

Sample

100ng to 1pg extracted RNA

or 5ul VTM

(see IFU for VTM compatibility)

Dyes/Cycles

N1= FAM, N501 Wildtype=VIC, N501Y Variant=Cy5, RNaseP=TexasRed. Amplification 40 cycles, LoD 5-10 copies

PERFORMANCE EVALUATION

Contrived Clinical Samples

N501Y Variant

N501Y Variant result. HEX (wild type) shows no amplification, while Quasar 670 (N501Y variant) is amplified.

N501 Wildtype

N501 Wild type result. HEX (wild type) shows amplification, while Quasar 670 (N501Y variant) shows no amplification.

N1

N1 detection and control RNase P

ORDERING INFORMATION

For Research Use Only. CLIA labs must file an LDT or EUA.

No- it is RUO/LDT

CDC, Pyramid, Copan, Saline, PBS, Mawi-El

5ul

The N1 utilizes CDC primer/probe sequences and is designed to allow the assay to be used both as a primary screening tool (positive/negative) for SARS-CoV-2 and confirmatory of previously positive samples (variant identification).

SARS-CoV-2 A(23063)T

The assay uses standard channels FAM, HEX, Cy5, ROX.

Product Disclaimer

Download SDS Sheets

DOING OUR PART

By highlighting threatened or endangered species, SeqOnce hopes to draw attention to conservation efforts. As biologists, we believe humanity is better served when ecologies are maintained and there is diversity of life.

SeqOnce supports protecting and conserving endangered and threatened species.

Go to Top