AzureSeq SARS-CoV-2 E484K

ONE STEP UNIVERSAL RT-qPCR KIT
SARS-CoV-2 E484K VARIANT DETECTION

Rapidly detect the E484K variant without sequencing while greatly increasing the number of samples screened. This assay detects the SARS-CoV-2 S gene (G23012A) variant E484K. This variant is reported in lineages SA B.1.351, and Brazil P.1.

TWO PROTOCOLS
Direct & Extracted

Multiplex – 3 targets per reaction well
Made in America
Research Use Only

Targets

S gene of SARS-CoV-2, RNaseP gene (control)

Sample

100ng to 1pg extracted RNA

or 5ul VTM

(see IFU for VTM compatibility)

Dyes/Cycles

E484 Wildtype=FAM, E484K Variant=HEX, RNaseP=TexasRed. Amplification 40 cycles, LoD 5-10 copies

PERFORMANCE EVALUATION

Contrived Clinical Samples

WT E484

SNP Genotyping

E484K

Results: Amplification of synthetic controls + Human RNA, and SNP genotyping of E484K

ORDERING INFORMATION

For Research Use Only. CLIA labs must file an LDT or EUA.

Product Disclaimer

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DOING OUR PART

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SeqOnce supports protecting and conserving endangered and threatened species.

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