AzureSeq: SARS-CoV-2 Variant Assay: E484K, N501Y, K417

UNIVERSAL RT-qPCR KIT
VARIANT OF CONCERN DETECTION

Rapidly detect the SARS-CoV-2 E484K, N501Y, and K417 variants without sequencing and greatly increase samples screened. These variants of concern are reported in a number of lineages including UK B.1.1.7 , SA B.135.1, and Brazil P.1.

Detect Variants of Concern in Less Than One Hour

Multiplex – 5 targets per reaction well
FAM/HEX/Cy5/Texas Red

Made in America
Research Use Only

Targets

SARS-CoV-2 E484, N501Y, K417, RNaseP gene

Sample

100ng to 1pg extracted RNA (5ul)

Dyes/Cycles

Wild type K417= FAM, N501Y=Cy5, E484K= HEX, RNaseP=TexasRed. Amplification 40 cycles, LoD 5-10 copies

PERFORMANCE EVALUATION

Contrived Clinical Samples

WT K417, WT N501, E484K variant

FAM (K417) and HEX (E484K) shows amplification while Quasar670 (Cy5 N501Y variant) shows no amplification.

WT K417, N501Y Variant

HEX (E484K) shows no amplification while FAM (K417) and Quasar670 (Cy5 N501Y variant) shows amplification.

Controls

Wild type K417 and control
RNase P

ORDERING INFORMATION

For Research Use Only

SARS-CoV-2 A(23063)T, G(23012)A, A(22812).

No- it is RUO/LDT

5ul

The assay uses standard channels FAM, HEX, Cy5, ROX.

Product Disclaimer

Download SDS Sheets

DOING OUR PART

By highlighting threatened or endangered species, SeqOnce hopes to draw attention to conservation efforts. As biologists, we believe humanity is better served when ecologies are maintained and there is diversity of life.

SeqOnce supports protecting and conserving endangered and threatened species.

Go to Top