ONE STEP UNIVERSAL RT-qPCR KITS LYOPHILIZED FORMATS
Get high multiplex performance in an American-made product. Excellent sensitivity with CDC-recommended primers. All enzymes are synthesized in the United States. Fast turn-around times for customization that fits your workflow and format.
Direct: No RNA Extraction Indirect: Use CDC Recommended Extraction
RUO, Not For Diagnostics CLIA Labs Must File LDT or EUA
AzureSeq was determined to be compatible with RNA extraction workflow at concentrations at or near LoD. Pooled negative OP swabs were spiked with genomic RNA. Input volume for extraction was 250 μl and elution volume was 80 μl. A 5 μl aliquot of the eluate was added to the PCR reaction.
Wet vs. Lyophilized
AzureSeq (wet) and AzureSeq Lyo (lyophilized) show comparable results using CDC specified primers and probes for nCoV2 N1 and N2 and RPP controls using CDC recommended testing protocol.
Detection on nCoV2 N1 and N2 genes: 50 copies of genomic RNA added in a 20 μl reaction. Reaction set-up and RT-qPCR protocol were in accordance with CDC guidance.
AzureSeq LoD Data
Legend: Negative nasopharyngeal swabs were spiked with heat-killed SARS-CoV-2 virus at the labeled concentration in copies/uL in starting sample media (saline). Samples were heated at 95C for 5 min, then 5uL of heat-inactivated sample was added to reactions containing 1X AzureSeq CoV-2 reagents. The final reaction volumes were 20ul. Samples were run on a BioRad CFX96 using default ramp rates.
Legend: Negative nasopharyngeal swabs were spiked with heat-killed SARS-CoV-2 virus at the labeled concentration in copies/uL (20 replicates each conc) starting sample media (saline). Samples were heated at 95C for 5 min, then 5uL of heat-inactivated sample was added to reactions containing 1X AzureSeq CoV-2 reagents. The final reaction volumes were 20ul. Samples were run on a BioRad CFX96 using default ramp rates.
AzureSeq Lyo and AzureSeq Plus Lyo is for RUO only. CLIA labs must file an LDT or EUA.
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