AZURESEQ SARS-COV-2 VARIANT DETECTION

ONE STEP UNIVERSAL RT-qPCR Kit
Select Panels & Mutations

Rapidly detect variants in SARS-CoV-2 without sequencing. Decrease costs, increase samples screened, and dramatically improve time to result. Assays are available as panels.

Detect Variants of Concern
in Less Than One Hour

Single-Tube RT-qPCR Detection of Multiple Variants

Made in America
Research Use Only

Enable daily processing of hundreds of samples.

Accurate detection with reduced complexity, time and waste.

Low copy number (5-10 copies) delivers a reliable, highly sensitive one-step RT-qPCR solution for rapid testing.

Compatible with CDC recommended RNA extraction kits and qPCR instruments.

AzureSeq SARS-CoV-2
Variant Panel v1.0

Multiplex Detection of
Alpha, Beta, and Delta Variants

This multiplex RT-qPCR assay differentiates the Alpha, Beta, and Delta SARS-CoV-2 Variants of Concern utilizing the spike protein mutations L452R, N501Y, and the wild type allele at K417. The assay uses RNase P as the sample control gene.

Targets

SARS-CoV-2: L452R, N501Y & 417Wt and human RNaseP gene

Sample

100 ng to 1 pg extracted RNA (5ul)

Dyes

417Wt = FAM
N501Y = Cy5
L452R = HEX
RNaseP = TexasRed

Parameters

Amplification 40 cycles, LoD 5-10 copies

PERFORMANCE EVALUATION

Contrived Clinical Samples

WT K417, WT N501, L452R variant

FAM (K417) and HEX (L452R) shows amplification while Quasar670 (Cy5 N501Y variant) shows no amplification.

WT K417, N501Y Variant

HEX (L452R) shows no amplification while FAM (K417) and Quasar670 (Cy5 N501Y variant) shows amplification.

Controls

Wild type K417 and control RNase P.

CONTACT US FOR MORE INFORMATION

DETECTING VARIANTS OF CONCERN

AzureSeq SARS-Cov2 Variant Panel v1.0 can identify all of the below variants of concern.
Click each tab to learn more.

First Identified: UK

Spike Protein Substitution

  • 69del, 70del, 144del, (E484K*), (S494P*), N501Y, A570D, D614G, P681H, T716I, S982A, D1118H (K1191N*)

AzureSeq SARS-CoV-2 Variant Panel v1.0

  • RNase P = Pos
  • L452R = Neg
  • N501Y = Pos
  • 417Wt = Pos

Potential Attributes Listed by CDC

  • ~50% increased transmission
  • Potential increased severity based on hospitalizations and case fatality rates
  • No impact on susceptibility to EUA monoclonal anybody treatments
  • Minimal impact on neutralization by convalescent and post-vaccination sera

First Identified: South Africa

Spike Protein Substitution

  • D80A, D215G, 241del, 242del, 243del, K417N, E484K, N501Y, D614G, A701V

AzureSeq SARS-CoV-2 Variant Panel v1.0

  • RNase P = Pos
  • L452R = Neg
  • N501Y = Pos
  • 417Wt = Neg

Potential Attributes Listed by CDC

  • ~50% increased transmission
  • Significantly reduced susceptibility to the combination of bamlanivimab and etesevimab monoclonal anybody treatment, but other EUA monoclonal anybody treatments are available
  • Reduced neutralization by convalescent and post-vaccination sera

First Identified: India

Spike Protein Substitution

  • T19R, (V70F*), T95I, G142D, E156-, F157-, R158G, (A222V*), (W258L*), (K417N*), L452R, T478K, D614G, P681R, D950N

AzureSeq SARS-CoV-2 Variant Panel v1.0

  • RNase P = Pos
  • L452R = Pos
  • N501Y = Neg
  • 417Wt = Neg

Potential Attributes Listed by CDC

  • Increased transmissibility
  • Potential reduction in neutralization by some EUA monoclonal anybody treatments
  • Potential reduction in neutralization by post-vaccination sera

ORDERING INFORMATION

SKU# 20 ul Reactions Content
ASD-ENK-Variant-200 200 In Solution: InhibiTaq Plus, Hot Start Mastermix, target specific primers and probes, DTT, UDG
ASD-ENK-Variant-1000 1000
ASD-ENK-Variant-2000 2000
ASD-ENK-Variant-Custom Custom

For Research Use Only

Contact Us for More Information

SARS-CoV-2 A(23063)T, G(23012)A, A(22812).

No- it is RUO/LDT

5ul

The assay uses standard channels FAM, HEX, Cy5, ROX.

Product Disclaimer

Download SDS Sheets

DOING OUR PART

By highlighting threatened or endangered species, SeqOnce hopes to draw attention to conservation efforts. As biologists, we believe humanity is better served when ecologies are maintained and there is diversity of life.

SeqOnce supports protecting and conserving endangered and threatened species.

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